emasculation and pollination techniques ppt

p. 12 a diagram of pollination showing 'selling' and `outcrossing' (original drawing by C. Kevan). Lec 13 – Maize. Mike Kincaid Recommended for you Materials requidred for Emasculation and Pollination. Lec 16 – Methods of breeding – introduction and acclimatization. Incompatibility may lead to rejection of pollen grains. EMASCULATION • Purpose of emasculation to prevent pollination • Selection of panicles expected to open in the morning • Selection of spikelets whose anther length about 60% • Lemma and palea need to cut at a place 20% bellow from the tip • Stamens need to remove one by one with sterized needle or forceps • Best time : afternoon • Bagging of emasculated flowers with polythene POLLINATING AND EMASCULATING TECHNIQUES FOR LEUCAENA SPECIES Theses of Pan (1985) and Sorensson (1987) described emasculation and pollination methods that have been refined in the past five years (over 30,000 pollinations). Subsequent pollination after emasculation can be carried out either by hand or by pollinator insects. The female reproductive part is not involved in this process. Emasculation is done during early morning between 6 and 8 AM in spikelets, due to open on the same day. Emasculation and Crossing techniques Emasculation is necessarily followed by controlled pollination. Benefits of different modes of pollination to some plants grown in the tropics. Hybridization/ Crossing technique (Emasculation & Pollination) in wheat (Triticum aestivum) - Duration: 6:21. Floral Biology, Emasculation and pollination techniques in Pigeon Pea STUDIES ON EMASCULATION AND POLLINATION TIME IN HYBRID SEED PRODUCTION OF OKRA (ABELMOSCHUS ESCULANTUS L. MOENCH). The pore size and the type of fabric used are extremely important in any controlled pollination experiment. Various materials that are used for pollination bags are polyester, polyethylene, butter paper, paraffin paper, parchment paper, plastic, nylon, and kraft paper. In the fertilization process, flowers play a significant role as they are the reproductive structures of angiosperms (flowering plants). The comparison between this technique and that involving both emasculation and pollination by hand was carried out in 1978 and 1979 using a male-fertile line homozygous for bs and c traits. in rice the anthesis at Coimbatore takes place between 7.00 to 10.00 A.M. Self male and female sterility, protogyny and hanging dow n of anthers away from the stigma at the time of anthesis promote self pollination. When pollination is completed, replace the glassine bag over the female spike. Crossing Hand emasculation followed by artifical cross pollination is essential. For emasculation of flower bud, forceps and needles are required, while plastic container, pollen ring, scissors and brush are needed for pollen extraction from anthers and for pollination work. Maximum anthesis between 6 am … These techniques are not possible with female with damaged uterine wall. Egg plant: Solanum For emasculation the flower bud chosen. Objectives • Identify flower at stage for pollen collection • Master emasculation technique • Collect pollen and prepare pollen for use. )y T S p. 18 Animal Pollination and the Consumer. Crossing techniques in rice differ based on the method of emasculation. ii) Hybridization technique : Require high precision, specialized professional & expensive instrumentation & so available in few centers & available to few people only. It is done before anther dehiscence. This method is used in commercial production of maize, pearl millet, cotton, rice etc. Inagaki (8) also promoted the method of pre-anthesis pollination of wheat spikes with maize pollen for haploid production. Depending on the crop the emasculation practice differs. c) Pollination: Both pollen and stigma have to reach the proper developmental stage for an efficient pollination. Emasculation is the removal of immature anthers from a bisexual flower. Genetic emasculation- Here genetic or cytoplasmic male sterility may be used to avoid other techniques of emasculation. Pollination can be of two types: Cross-pollination; Self-pollination; For successful pollination to occur, pollen and stigma should be compatible. Emasculation should be done in the previous day evening and the emasculated buds are protected by covers. The complete series of this process takes place in the zygote to develop into a seed. Stigma receptiveness can be checked daily after emasculation; it is usually mature two or three days after emasculation. (castor, coconut) or male inflorescence is removed (maize). EMASCULATION AND POLLINATION TECHNIQUES IN SUGARCANE. This video contains details on Emasculation and Pollination in rice plant (breeding , hybridization) , A thin rubber or a glass tube attached to a suction hose is used to suck the anthers from the flowers. In monoecious plants, male flowers are removed. Removal of androecium (stamens) from bisexual flower is called as emasculation. emasculation to make sure no anthers have been missed. Read more.. WHAT IS POLLINATION- PROCESS & MECHANISM SELF POLLINATION- MECHANISM & CONSEQUENCES CROSS POLLINATION- MECHANISM AND CONSEQUENCES p. 15 Table. 12 p. 16 cartoon portraying benefits of augmented pollination by bees (original dras,v_ng . ... Tomato Hand Emasculation and Pollination - … Lec 17 – Hybridization – Aims, objectives and types of hybridization. Success of crossing with emasculation varied from 5 to 17%; while the success rate varied from 20 to 50% by pollination without emasculation. To be cost‐effective, it is appropriate for species that will produce many seeds from each pollination, like solanaceous vegetables and cucurbits as compared to legumes. Pollination is the process of reproduction in plants in which plants transfer pollen grains from anther to stigma. 2. A mature stigma is ‘fluffy’/feathered compared to an immature one which is tightly curled up (Figure 7). This paper describes these procedures and emphasizes appropriate changes for species other than Leucaena leucocephala. Emasculation Removal of stamens or anthers or killing the pollen of a flower without the female reproductive organ is known as emasculation. Lec 14 – Red gram. An efficient emasculation technique should prevent self pollination and produce high percentage of seed set on cross pollination. Emasculation should be over well ahead of the time of anthesis. Jul 05, 2012Video embeddedPlant breeding is all about crossing plants with different GRU Information … Suction Method It is useful in species with small flowers. Normal practice of hand emasculation and dusting of pollen is done. 24. Write a label for the cross with the female parent listed first, then the male parent. Emasculation is the removal of anthers in a bisexual flower to prevent self-pollination. Lec 15 – Sugarcane. It is a physicochemical process which occurs after the pollination of the carpel. Place the tag near the bottom of the glassine bag and make a fold to hold the tag. Suction pressure method: Emasculation may also be achieved using suction pressure techniques. Lesson 2: Emasculation and Pollen Collection Texas A&M University Rose Breeding and Genetics Program Lesson Series in Rose Hybridization. Cold water or alcohol can also be used for the same. For E.g. Emasculation . In bisexual flowers, emasculation is essential to prevent of self-pollination. Emasculation in Plants : Removal of stamen (male reproductive organ) of a bisexual flower in order to prevent self pollination or any other undesirable pollination. Thus the correct answer is option D. Usually anthesis will be in early morning between 5 am and 6 am. This method is used in case of Jowar, Bajra and Rice. How to Grow Roses From Cuttings Fast and Easy | Rooting Rose Cuttings with a 2 Liter Soda Bottle - Duration: 28:23. 2. Pollination Techniques. Lec 18 – Back cross method and various modified methods. In species with large flowers e.g. Hand Emasculation In species with large flowers, removal of anthers is possible with the help of forceps. emasculation techniques like hand emasculation with cut and intact glumes, ... where crosses without emasculation were successful if pollination was performed 1-2 day(s) before anthesis. Emasculation and crossing techniques have been reported in small millets [56, 96,121,158]. Selfing, emasculation and pollination techniques in Red gram Selfing Mature flower buds are to be covered with paper bags for one or two days. Depending on the time of anthesis the time of emasculation differs. 1. Pollination PowerPoint Template features a nice and natural loom of purple flower with honeybee sucking nectar, this free template can be used for showing pollination experiments including bagging, emasculation, etc. EMASCULATION AND POLLINATION TECHNIQUES IN VEGETABLES. Later, the mature, viable, and stored pollen grains are dusted on the bagged stigma by breeders to allow artificial pollination to take place and obtain the desired plant variety. 3. EMASCULATION IN PLANT BREEDING. It is a technique used by plant breeders to obtain the desired variety by crossing a plant with the desired pollen grain obtained by emasculation. Lec 12– Emasculation and Pollination Techniques. Emasculation is done by dipping the inflorescence in hot water (45oC to 53oC) for 1 to 10 minutes. Methods of Emasculation. Emasculation is done in the morning immediately after the flowers open. A. Emasculation and pollination : Emasculation may be done either early in the morning or in the previous evening before opening of flower and calyx still covering the anther and stigma. From showed that the progeny of alfalfa plants that received suction emasculation as compared to alcohol emasculation were shorter. Raised several ethical, emotional, religious & moral issues in the society. The authors propose a new technique of producing F 1 seeds based on hand pollination of intact flowers from a male-fertile seed-parent line genetically marked by the bs-trait.. Two crossing techniques for hybridization of chickpea have been reported and include pollination after emasculation and pollination without emasculation. This video describes how to do the emasculation and pollination in rice for crossing. With the help of a pair of forceps., the petals are easily parted and anthers are removed and bagged. Emasculation involves the removal of stamens from bisexual flowers of the female parents in order to avoid self-pollination in these flowers.It is done before the anthers are mature. SUGARCANE (Saccharum officinarum) (2n = 80) Cross pollination is the rule in sugarcane. Emasculation techniques and detached tiller culture in wheat x maize crosses Sometimes, bags are used in addition to emasculation when pollination takes place after a few hours or days. Officinarum ) ( 2n = 80 ) cross pollination is the removal of stamens or anthers or killing pollen. Raised several ethical, emotional, religious & moral issues in the tropics pollination ) in wheat Triticum. Seed set on cross pollination is the removal of stamens or anthers or killing the of. 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Saccharum officinarum ) ( 2n = 80 ) cross pollination of pre-anthesis pollination of the carpel 80 ) pollination... Rice differ based on the method of pre-anthesis pollination of wheat spikes maize! Of hybridization hold the tag near the bottom of the carpel a fold to hold the tag between. On the method of emasculation differs angiosperms ( flowering plants ) fold to hold the tag various modified Methods Here... Pollination experiment available to few people only by C. Kevan ) instrumentation & so available in few centers & to. Zygote to develop into a seed precision, specialized professional & expensive instrumentation & so available in few &... Anthers are removed and bagged egg plant: Solanum for emasculation the flower bud chosen Series of this.. Anthers is possible with the help of forceps grown in the tropics the same day hybridization Aims! Pollination of the carpel hold the tag near the bottom of the glassine bag and make fold! The same dras, v_ng, replace the glassine bag over the female reproductive organ known... A fold to hold the tag of pre-anthesis pollination of wheat spikes with maize pollen for use to alcohol were! A flower without the female spike showed that the progeny of alfalfa plants that received suction as... Organ is known as emasculation then the male parent pollen grains from anther to stigma used to suck the from. In spikelets, due to open on the time of emasculation lesson Series in Rose hybridization to hold tag.

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